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tularemia examination, diagnosis of Tularemia

tularemia examination, diagnosis of Tularemia

Common examinations for tularemia

Inspection Name Inspection Site Inspection Department Inspection Function
A complete blood count blood vessels blood and health care complete blood count ...
Blood and bone marrow bacteria culture blood vessels and bone marrow pathology department of blood and bone marrow...
One, check

1. Skin test

(1) The disadvantage of this method is that the clinical diagnosis cannot rule out the possibility of having the disease in the past, and it is mainly used for epidemiological investigations.

(2) The specific method is as follows: prepare turamycin with diluted dead bacteria suspension or purified antigen, inoculate 0.1ml of bacterin in the skin of the forearm, and check the results for 12-24 hours. If there is redness and swelling, it is a positive reaction. Reactions will appear on the 3rd to 5th sick days.

2. Serological test

(1) Serum agglutination test: antibody positive begins in the second week of the disease course, and the titer reaches a peak after 1 to 2 months, up to 1:1280, and the antibody can last for several years. Agglutination titer of 1:100 or higher or a 4-fold increase in antibody titers between the acute and convalescent sera have diagnostic significance, and cross-reaction with Brucella can be ruled out;

(2) Reverse indirect hemagglutination test: It has the characteristics of early and rapid diagnosis, and the results can be obtained after 1 to 2 hours. (3) Immunofluorescence antibody method: It can be used for early and rapid diagnosis, and the results can be obtained in 1 to 2 hours. It has good specificity and sensitivity. .

3. Blood examination

After examination, it can be found that there is an increase in white blood cells (10-12) × 109/L at the beginning of the disease, showing an increase in neutrophils. In the late course of the disease, white blood cells decreased, and the ratio of lymph to monocytes increased, with rod-shaped neutrophils.

4. Bacterial culture

Local ulcer secretions, enlarged lymph nodes, sputum, gastric washing fluid or acute blood samples are cultured on glucose-cystine-blood agar medium, and pathogenic bacteria can be isolated after 48h.

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