examination of dermatitis blastomyces, diagnosis of dermatitis blastomyces
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- Fungal cultureWhole bodyDepartment of Dermatology and Health CareFungal culture examination...
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1. Direct microscopic examination of sputum, pus, bone marrow, blood, cerebrospinal fluid, pleural effusion, urine, biopsy or cadaver tissue samples for direct examination.
2. Fungi were cultured on sandcastle agar at room temperature, and began to grow as yeast-like membrane, and then fine spiny hyphae appeared in the center, which gradually increased and formed a central ring.
Histopathology: Epithelial cell-like granuloma or chronic suppurative necrosis and fibrosis can be seen. Depending on the disease, such as chronic epithelioma-like hyperplasia, while systemic infection can see suppuration, necrosis and many bacteria. The bacterium is about 8 ~ 12 μ m in diameter, sometimes up to 20 ~ 30 μ m in diameter, and its wall thickness is obvious, like double wall or birefringent, and its cytoplasm condenses from the cell wall to form a void. HE staining showed multinucleated cytoplasm. Primary lung infection is the first inflammatory cell reaction, many neutrophils can be seen, sometimes chronic granulomatous with suppurative area or epithelioid cell granuloma, which has many small abscesses containing neutrophils, fragments and a few giant cells, and thallus can be seen. If hyperplasia can be like tumor, it is helpful to diagnose only if microabscess is found.
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