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Not all serotypes are suitable, only serotypes are suitable after the following conditions:
(1) When used in seroepidemiological investigation of a specific serotype
(2) Characteristic clinical manifestations such as epidemic chest pain have been found, which clearly indicate that some specific antigens (such as group B virus) are used to detect antibodies; Or hand, foot and oral diseases are usually caused by Coxsackie A16 virus
(3) When the virus has been isolated, it can be used to determine the serotype
(4) When an epidemic caused by a single serotype virus is occurring,
Among all the serological tests, neutralization test is the most specific method to identify the isolated virus serotype.
2. Peripheral hemogram
It shows that the total number of white blood cells is normal or slightly increased.
3. Virus isolation method
This method is the main method for diagnosis. Its advantages are economy, rapidity and accuracy. Thus, the difficulty of various serotypes encountered by serological methods is avoided.
It is worth noting that:
(1) The positive rate of virus isolated from feces is the highest, and it can still be positive within 10 days after onset.
(2) Viruses can be isolated from throat swabs or gargles for respiratory tract infections.
(3) The positive rate of virus isolated from cerebrospinal fluid is low, but it is of great significance to diagnose.
(4) Other specimens including pleural effusion, pericardial effusion, urine, muscle biopsy tissue and autopsy nerve tissue can be sent for examination.
(5) Fecal specimens can be kept at 4 ℃ for many days, while other specimens should be kept below-7 ℃.
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